Journal: Experimental and Therapeutic Medicine

Article Title: Decreased expression of microRNA-223 promotes cell proliferation in hepatocellular carcinoma cells via the insulin-like growth factor-1 signaling pathway

doi: 10.3892/etm.2018.5929

Figure Lengend Snippet: miR-223 regulates IGF-1R expression in the Hep3B cell line. (A) The 224–231 of the IGF-1R 3′-untranslated region is the putative target site of miR-223. (B) Relative mRNA expression of IGF-1R in cells transfected with miR-223 mimic, miR-223 inhibitor and NC, compared with the BL. The expression of IGF-1R mRNA was normalized to GAPDH. (C) Expression of IGF-1R protein detected by western blotting in cells transfected with miR-223 mimic, miR-223 inhibitor or NC, compared with the BL group. GAPDH was used as a loading control. Quantified data are presented as the mean ± standard deviation. **P<0.01 vs. BL. BL, blank control; NC, negative control RNA; mimic, miR-223 mimic; inhibitor, miR-223 inhibitor; IGF-1R, insulin-like growth factor-1 receptor; miR-223, microRNA-223.

Article Snippet: The primary antibodies used were as follows: Rabbit polyclonal to IGF-1R (G1155; 1:1,000; cat. no. BS1182; Biocompare, South San Francisco, CA, USA); rabbit polyclonal to ERK 1/2 (1:1,000; cat. no. ab196883); rabbit polyclonal to phosphorylated (p)-ERK 1/2 (1:1,000; cat. no. ab214362); rabbit polyclonal to Akt (1:1,000; cat. no. ab8805); rabbit polyclonal to p-Akt (1:1,000; cat. no. ab38449); and rabbit polyclonal to GAPDH (1:1,000; cat. no. ab9485; all Abcam, Cambridge, MA, USA).

Techniques: Expressing, Transfection, Western Blot, Control, Standard Deviation, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: Decreased expression of microRNA-223 promotes cell proliferation in hepatocellular carcinoma cells via the insulin-like growth factor-1 signaling pathway

doi: 10.3892/etm.2018.5929

Figure Lengend Snippet: miR-223 regulates downstream signaling of the IGF-1 pathway. The expression of Akt and ERK were measured in cells transfected with miR-223 mimic, miR-223 inhibitor or NC and compared with the BL group. (A) The expression of total Akt, total ERK, and their active forms p-Akt and p-ERK were measured by western blot analysis. (B) Relative mRNA level of Akt and ERK normalized to GAPDH. Quantified data are presented as the mean ± standard deviation. **P<0.01 vs. BL. BL, blank control; NC, negative control RNA; mimic, miR-223 mimic; inhibitor, miR-223 inhibitor; IGF-1R, insulin-like growth factor-1 receptor; miR-223, microRNA-223; p-, phosphorylated; Akt, protein kinase B; ERK, extracellular-signal regulated kinase.

Article Snippet: The primary antibodies used were as follows: Rabbit polyclonal to IGF-1R (G1155; 1:1,000; cat. no. BS1182; Biocompare, South San Francisco, CA, USA); rabbit polyclonal to ERK 1/2 (1:1,000; cat. no. ab196883); rabbit polyclonal to phosphorylated (p)-ERK 1/2 (1:1,000; cat. no. ab214362); rabbit polyclonal to Akt (1:1,000; cat. no. ab8805); rabbit polyclonal to p-Akt (1:1,000; cat. no. ab38449); and rabbit polyclonal to GAPDH (1:1,000; cat. no. ab9485; all Abcam, Cambridge, MA, USA).

Techniques: Expressing, Transfection, Western Blot, Standard Deviation, Control, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: Decreased expression of microRNA-223 promotes cell proliferation in hepatocellular carcinoma cells via the insulin-like growth factor-1 signaling pathway

doi: 10.3892/etm.2018.5929

Figure Lengend Snippet: Correlation between miR-223 and IGF-1R expression in tumor tissues of patients with hepatocellular carcinoma. r=−0.965; P<0.001. miR-223, microRNA-223; IGF-1R, insulin-like factor 1 receptor.

Article Snippet: The primary antibodies used were as follows: Rabbit polyclonal to IGF-1R (G1155; 1:1,000; cat. no. BS1182; Biocompare, South San Francisco, CA, USA); rabbit polyclonal to ERK 1/2 (1:1,000; cat. no. ab196883); rabbit polyclonal to phosphorylated (p)-ERK 1/2 (1:1,000; cat. no. ab214362); rabbit polyclonal to Akt (1:1,000; cat. no. ab8805); rabbit polyclonal to p-Akt (1:1,000; cat. no. ab38449); and rabbit polyclonal to GAPDH (1:1,000; cat. no. ab9485; all Abcam, Cambridge, MA, USA).

Techniques: Expressing